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| Chemicals \ Wright-Giemsa |
Wright-Giemsa Stain Information / Comments
| Made from Certified Wright's and Giemsa stain, in a solution of methyl alcohol and glycerin.
Used as a rapid cellular stain for smears, such as Tzanck smears. It provides cytoplasm with a light blue stain, and nuclear material a dark blue. To obtain hues similar to that of H&E staining, Delasco's PMS stain is used in the same manner as Wright-Giemsa stain, but stains cytoplasm pinkish red, and nuclear material blue.
Usage:
Smear specimen onto slide. Heat fix by warming slide so that it is warm to the back of the hand. Dip in alcohol, 30% to 80%, then flood the slide. (If 30 to 80% alcohol is not available, Wright Giemsa contains nearly 90% alcohol and may be selffixing.) Allow the stain to remain on the slide for 30 to 60 seconds. Rinse slide gently with running water. Air dry, and cover with oil or slide adhesive, and coverslip and examine.
Hint:
Fresh vesicles provide better material. Old vesicles or pustules may not stain well, and show mostly degenerating cells.
Provided in easy to use, drop dispensing plastic containers in 1 and 2 oz.
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